HERBALISM: MORINGA OLEIFERA RESEARCH

Herbalism is a traditional medicinal or folk medicine practice based on the use of plants and plants extract.  Moringa Oleifera is a multifunctional plant.  It has been cultivated in tropical regions all over the world for high protein, vitamins and carbohydrate content of entire plant.  This research work was carried out to determine some effects of Moringa oleifera on the micro architecture of developing lungs of wistar rats, also on the average weight of the animals.

Source: en.wikipedia.org

            

Fifteen (15) wistar rats of both sexes of average weight between 20-30g of about 3 weeks old were divided into three groups of 5 animals each. Group 1 was the control group and received 2ml of distilled water each. Group 2 received 100mg/kg of the extract while group 3 received 200mg/kg of the extract.  The experiment was done for 6 weeks. After 6 weeks the animals were sacrificed using cervical dislocation.  The lungs were removed, weighed and fixed in 10% formolsaline for H&E staining.

            The results showed that there was significant difference in the feed intake of animals in group 2 compared to the control group from the 4^th week of administration, while there was significant difference in the feed intake of the animals in group 3 compared to the control group throughout the 6 weeks of administration.

            Histological findings showed that administration of 

Moringa oleifera has positive effect on the histological architecture of the developing lungs.

            In conclusion, this study showed that Moringa oleifera increases feed intake and the increase was dose dependent. It also showed that Moringa Oleifera does not have significant effect on the weight of the animals.

IRON OXIDE NANOPARTICLES

Iron oxide nanoparticles can be used for medical imaging, disease diagnosis, drug delivery, cancer treatment, gene therapy and other cases. These particles accumulate in liver cells and lead to oxidative stress with generation of reactive oxygen species. 

Source: www.news.softpedia-static.com

This research is aimed at investigating the adverse effect of ferrous oxide nanoparticles on liver of wistar rats. In this research 35 male wistar rats were used for experiment, and they were grouped into four groups. One group was control and other three experimental groups were exposed to ferrous oxide nanoparticle at different exposure (24hours, 48hours and 72 hours).

All the experimental animals were sacrificed by cervical dislocation. Immediately after the experimental animals were sacrificed, they were dissected and the liver was harvested and fixed in 10% Formosaline. The tissues were later processed for histological techniques and stained with hematoxylin and eosin (H&E). The histological analysis of control group shows normal sinusoids, hepatocytes, central vein. The result reveals that the administration of ferrous oxide nanoparticle affects the sinusoids, central vein and the hepatocytes. It also showed hemorrhage on the central vein, necrosis, disorganized sinusoids, deformed bile duct, ruptured hepatic artery and damaged hepatocytes. The result of this research reveals that ferrous oxide nanoparticles affects the liver and causes severe damage to the liver. Also there was weight decrease after administration of the experimental animals.

THE EFFECT OF FERROUS OXIDE NANOPARTICLES ON TESTIS OF WISTAR RATS

This study is aimed at investigating the effect of ferrous oxide nanoparticles on testis of wistar rats. In this research 35 wistar rats were used as experimental animals, grouped into four and designated as 24hours, 48hours, 72hours and control were acclimatized for two weeks. 

Source: english.farsnews.com

After acclimatization, each group of the experimental animals was sub-divided into 5 low dose and 5 high dose except the control. 30 wistar rats were administered intravenously at low dose of 5mg/kg and high dose of 10mg/kg while the 5 control had access to only feeds and water. The histological analysis of the testis of control group shows normal seminiferous tubules, spermatogonia, sertoli cells and leydigs cells. The result reveals that the administration of ferrous oxide nanoparticle affects the seminiferous tubules, spermatogonia, sertoli cells, lumen of the seminiferous tubules, basement membrane and leydig cells of most of the groups especially at high dose. The result of low dose of 24hours and 48hours group shows scanty sertoli cells, some deformed seminiferous tubules and few spermatogonia. The result of   high dose of 24hours, 48hours, 72hours and low dose of 72hours group shows highly deformed seminiferous tubules, distorted basement membrane, enlarged lumen of the seminiferous tubules and degenerating germ cells. There is hemorrhage in the seminiferous tubules of 48hours high dose group. The result of this study reveals that the administration of ferrous oxide nanoparticles affects the testis and will reduce fertility.